Genotyping and primer details


Genotyping and primer details for 241G12 - At2g47490
Line ID 241G12
Locus At2g47490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19489307 of line 241G12 Chr2:19489307
   
Insertion allele amplicon 8474 - BG35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BG35: 5'-TTTCCCACATATGAGATGATCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19488762
   
wt amplicon (over InsPos) 21ep - BG35
wt amplicon start Chr2:19488762
wt amplicon end Chr2:19489900
wt amplicon size 1139 bp
Locus specific primer BG35: 5'-TTTCCCACATATGAGATGATCAAA-3'
2nd locus specific primer 21ep: 5'-AATGGGTGAAACCGTAAGTAACAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11