Genotyping and primer details


Genotyping and primer details for 257E09 - At4g24450
Line ID 257E09
Locus At4g24450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12638748 of line 257E09 Chr4:12638748
   
Insertion allele amplicon 8474 - BM13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BM13: 5'-TATTTGCAGCAACCAAATAAGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12638155
   
Insertion allele amplicon 8474 - BM14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BM14: 5'-TTACCTTAGATGGAGAAAGGAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12639144
   
wt amplicon (over InsPos) BM14 - BM13
wt amplicon start Chr4:12638155
wt amplicon end Chr4:12639144
wt amplicon size 990 bp
Locus specific primer BM13: 5'-TATTTGCAGCAACCAAATAAGAGA-3'
2nd locus specific primer BM14: 5'-TTACCTTAGATGGAGAAAGGAACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11