Genotyping and primer details


Genotyping and primer details for 277A11 - At1g30670
Line ID 277A11
Locus At1g30670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10879493 of line 277A11 Chr1:10879493
   
Insertion allele amplicon 8474 - ZZ68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ZZ68: 5'-TACATCACAGAAGCTTCCAACATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10879885
   
wt amplicon (over InsPos) 08si - ZZ68
wt amplicon start Chr1:10879008
wt amplicon end Chr1:10879885
wt amplicon size 878 bp
Locus specific primer ZZ68: 5'-TACATCACAGAAGCTTCCAACATT-3'
2nd locus specific primer 08si: 5'-GAAGTCATGAAGTGTGTCCAAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11