Genotyping and primer details


Genotyping and primer details for 278E09 - At4g36220
Line ID 278E09
Locus At4g36220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17138656 of line 278E09 Chr4:17138656
   
Insertion allele amplicon 8474 - MM43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MM43: 5'-GTTCCTCCAAACATAACGTCCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17138185
   
wt amplicon (over InsPos) 26km - MM43
wt amplicon start Chr4:17138185
wt amplicon end Chr4:17139227
wt amplicon size 1043 bp
Locus specific primer MM43: 5'-GTTCCTCCAAACATAACGTCCTAA-3'
2nd locus specific primer 26km: 5'-TTGGAGACAGATGAGAAAAGTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11