Genotyping and primer details


Genotyping and primer details for 293D12 - At1g21410
Line ID 293D12
Locus At1g21410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7498741 of line 293D12 Chr1:7498741
   
Insertion allele amplicon 8474 - JD03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JD03: 5'-CAGCAAAAGCTTGAAAATTACAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7498398
   
wt amplicon (over InsPos) 04dw - JD03
wt amplicon start Chr1:7498398
wt amplicon end Chr1:7499009
wt amplicon size 612 bp
Locus specific primer JD03: 5'-CAGCAAAAGCTTGAAAATTACAGA-3'
2nd locus specific primer 04dw: 5'-CGAAATAGTACAAGACAAACCGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11