Genotyping and primer details


Genotyping and primer details for 295C05 - At3g11630
Line ID 295C05
Locus At3g11630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3673487 of line 295C05 Chr3:3673487
   
Insertion allele amplicon 8474 - NR58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NR58: 5'-GTACTCTTTGCTGAGTTTTGGGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3673922
   
wt amplicon (over InsPos) 03fd - NR58
wt amplicon start Chr3:3673063
wt amplicon end Chr3:3673922
wt amplicon size 860 bp
Locus specific primer NR58: 5'-GTACTCTTTGCTGAGTTTTGGGTC-3'
2nd locus specific primer 03fd: 5'-TACCCATTGGACTTTACTTTCGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11