Genotyping and primer details


Genotyping and primer details for 299G09 - At1g52410
Line ID 299G09
Locus At1g52410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19521846 of line 299G09 Chr1:19521846
   
Insertion allele amplicon 8474 - TS03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer TS03: 5'-AAGGTGAATTTTTGTCTTTGCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19521396
   
wt amplicon (over InsPos) 08em - TS03
wt amplicon start Chr1:19521396
wt amplicon end Chr1:19522424
wt amplicon size 1029 bp
Locus specific primer TS03: 5'-AAGGTGAATTTTTGTCTTTGCTGT-3'
2nd locus specific primer 08em: 5'-AAGCATACTTTTTCTCTTTGCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11