Genotyping and primer details


Genotyping and primer details for 310H12 - At2g29700
Line ID 310H12
Locus At2g29700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12697592 of line 310H12 Chr2:12697592
   
Insertion allele amplicon 8474 - 15fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15fp: 5'-TGTGATCATAATCGAGAACCTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12698000
   
wt amplicon (over InsPos) 07gl - 15fp
wt amplicon start Chr2:12697088
wt amplicon end Chr2:12698000
wt amplicon size 913 bp
Locus specific primer 15fp: 5'-TGTGATCATAATCGAGAACCTCAG-3'
2nd locus specific primer 07gl: 5'-AATAAAAATTGGTTCAGATGGACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11