Genotyping and primer details


Genotyping and primer details for 320A06 - At2g26770
Line ID 320A06
Locus At2g26770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11404008 of line 320A06 Chr2:11404008
   
wt amplicon (over InsPos) 12bd - 20aq
wt amplicon start Chr2:11403605
wt amplicon end Chr2:11404330
wt amplicon size 726 bp
Locus specific primer 20aq: 5'-TAGTGTTGGCTTTGTTGATGAGAC-3'
2nd locus specific primer 12bd: 5'-AGTATGAAACTGATGTGTTGCAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11