Genotyping and primer details


Genotyping and primer details for 322H06 - At2g31660
Line ID 322H06
Locus At2g31660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13470234 of line 322H06 Chr2:13470234
   
Insertion allele amplicon 8474 - CK32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CK32: 5'-TCTGGAGTCCATTCATTTCTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13470741
   
wt amplicon (over InsPos) 17em - CK32
wt amplicon start Chr2:13469958
wt amplicon end Chr2:13470741
wt amplicon size 784 bp
Locus specific primer CK32: 5'-TCTGGAGTCCATTCATTTCTTCTT-3'
2nd locus specific primer 17em: 5'-AACTATGTGGAGGATTTGTTGACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11