Genotyping and primer details


Genotyping and primer details for 329F04 - At2g35530
Line ID 329F04
Locus At2g35530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14922984 of line 329F04 Chr2:14922984
   
Insertion allele amplicon 8474 - DR11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DR11: 5'-TGTTGAACGAAGAAAACACAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14923568
   
wt amplicon (over InsPos) 02em - DR11
wt amplicon start Chr2:14922714
wt amplicon end Chr2:14923568
wt amplicon size 855 bp
Locus specific primer DR11: 5'-TGTTGAACGAAGAAAACACAAATC-3'
2nd locus specific primer 02em: 5'-GCACTTGCTTTCCATACATCATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11