Genotyping and primer details


Genotyping and primer details for 345F09 - At3g07940
Line ID 345F09
Locus At3g07940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2529512 of line 345F09 Chr3:2529512
   
Insertion allele amplicon 8474 - 10gz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10gz: 5'-CAGCACAATATTTATTCCCAGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2529913
   
wt amplicon (over InsPos) 08hv - 10gz
wt amplicon start Chr3:2528962
wt amplicon end Chr3:2529913
wt amplicon size 952 bp
Locus specific primer 10gz: 5'-CAGCACAATATTTATTCCCAGGTT-3'
2nd locus specific primer 08hv: 5'-AAAATCACAAAATCGAAAATCCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11