Genotyping and primer details


Genotyping and primer details for 355H08 - At3g25250
Line ID 355H08
Locus At3g25250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9196605 of line 355H08 Chr3:9196605
   
Insertion allele amplicon 8474 - FQ53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FQ53: 5'-ATTATTCAACCCCTTAACCCATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9196907
   
Insertion allele amplicon 8474 - KP06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP06: 5'-CTACAAATCTAGCTCCAAGAACGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9196195
   
wt amplicon (over InsPos) 08fd - KP06
wt amplicon start Chr3:9196195
wt amplicon end Chr3:9197046
wt amplicon size 852 bp
Locus specific primer KP06: 5'-CTACAAATCTAGCTCCAAGAACGC-3'
2nd locus specific primer 08fd: 5'-AGGTCCAACGATAATTTGAGTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11