Genotyping and primer details


Genotyping and primer details for 357B06 - At3g66654
Line ID 357B06
Locus At3g66654 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2089773 of line 357B06 Chr3:2089773
   
Insertion allele amplicon 8474 - JK33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JK33: 5'-CCTGTTTGCTTGCTTAAGTTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2089196
   
wt amplicon (over InsPos) 10si - JK33
wt amplicon start Chr3:2089196
wt amplicon end Chr3:2090129
wt amplicon size 934 bp
Locus specific primer JK33: 5'-CCTGTTTGCTTGCTTAAGTTTCTT-3'
2nd locus specific primer 10si: 5'-CAAACCTGTACTACATCCTCTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11