Genotyping and primer details


Genotyping and primer details for 369A04 - At3g22840
Line ID 369A04
Locus At3g22840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8084964 of line 369A04 Chr3:8084964
   
Insertion allele amplicon 3144 - CT32
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CT32: 5'-TTTTTAACCCGAAGTTTCAAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8084489
   
Insertion allele amplicon 8474 - DE24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DE24: 5'-GCTGAGGTACGTTATTCTCCACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8085307
   
wt amplicon (over InsPos) DE24 - CT32
wt amplicon start Chr3:8084489
wt amplicon end Chr3:8085307
wt amplicon size 819 bp
Locus specific primer CT32: 5'-TTTTTAACCCGAAGTTTCAAAATG-3'
2nd locus specific primer DE24: 5'-GCTGAGGTACGTTATTCTCCACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11