Genotyping and primer details


Genotyping and primer details for 391G12 - At3g02850
Line ID 391G12
Locus At3g02850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:621036 of line 391G12 Chr3:621036
   
Insertion allele amplicon 8474 - BE23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE23: 5'-TCGTTTGCTTTCATTCCTTAACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:621839
   
wt amplicon (over InsPos) 12fw - BE23
wt amplicon start Chr3:620928
wt amplicon end Chr3:621839
wt amplicon size 912 bp
Locus specific primer BE23: 5'-TCGTTTGCTTTCATTCCTTAACTT-3'
2nd locus specific primer 12fw: 5'-GTCAAGCCTTAGAATCCGACATAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11