Genotyping and primer details


Genotyping and primer details for 394E01 - At2g16640
Line ID 394E01
Locus At2g16640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7212820 of line 394E01 Chr2:7212820
   
Insertion allele amplicon 8474 - MG01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MG01: 5'-CCTGAATTCTGTTAAGGCTTTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7213175
   
wt amplicon (over InsPos) 09fq - MG01
wt amplicon start Chr2:7212467
wt amplicon end Chr2:7213175
wt amplicon size 709 bp
Locus specific primer MG01: 5'-CCTGAATTCTGTTAAGGCTTTCAT-3'
2nd locus specific primer 09fq: 5'-TTCATCGAGATACTGCTTCTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11