Genotyping and primer details


Genotyping and primer details for 443F03 - At1g20330
Line ID 443F03
Locus At1g20330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7039092 of line 443F03 Chr1:7039092
   
Insertion allele amplicon 8474 - JH25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JH25: 5'-ACGTAGTCGGGATTACAATAAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7039611
   
wt amplicon (over InsPos) 54dy - JH25
wt amplicon start Chr1:7038472
wt amplicon end Chr1:7039611
wt amplicon size 1140 bp
Locus specific primer JH25: 5'-ACGTAGTCGGGATTACAATAAACG-3'
2nd locus specific primer 54dy: 5'-AGCAATAATTCAAAATAACCACGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11