Genotyping and primer details


Genotyping and primer details for 446D11 - At2g32440
Line ID 446D11
Locus At2g32440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13777673 of line 446D11 Chr2:13777673
   
Insertion allele amplicon 8474 - NE16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NE16: 5'-AACCAAATTTGAAGCTAAACCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13777979
   
wt amplicon (over InsPos) 23ep - NE16
wt amplicon start Chr2:13777353
wt amplicon end Chr2:13777979
wt amplicon size 627 bp
Locus specific primer NE16: 5'-AACCAAATTTGAAGCTAAACCAGA-3'
2nd locus specific primer 23ep: 5'-TCAAGATGGGAGGTACGTAGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11