Genotyping and primer details


Genotyping and primer details for 453H07 - At1g13820
Line ID 453H07
Locus At1g13820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4736528 of line 453H07 Chr1:4736528
   
Insertion allele amplicon 8474 - JH15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JH15: 5'-GTTACGTTGTATCCTCCGCTAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4737212
   
wt amplicon (over InsPos) 38si - JH15
wt amplicon start Chr1:4736033
wt amplicon end Chr1:4737212
wt amplicon size 1180 bp
Locus specific primer JH15: 5'-GTTACGTTGTATCCTCCGCTAGTC-3'
2nd locus specific primer 38si: 5'-TTTTGCTCATTGTCTTGTTAGGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11