Genotyping and primer details


Genotyping and primer details for 481C08 - At2g02950
Line ID 481C08
Locus At2g02950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:856436 of line 481C08 Chr2:856436
   
Insertion allele amplicon 8474 - CF08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CF08: 5'-ACTGGAGATCCAAAAACTTCAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:855732
   
Insertion allele amplicon 8474 - CT33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CT33: 5'-ATTATTCTCCATTGATTGGCAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:856720
   
wt amplicon (over InsPos) CT33 - CF08
wt amplicon start Chr2:855732
wt amplicon end Chr2:856720
wt amplicon size 989 bp
Locus specific primer CF08: 5'-ACTGGAGATCCAAAAACTTCAAGA-3'
2nd locus specific primer CT33: 5'-ATTATTCTCCATTGATTGGCAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11