Genotyping and primer details


Genotyping and primer details for 498B04 - At3g11320
Line ID 498B04
Locus At3g11320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3548160 of line 498B04 Chr3:3548160
   
Insertion allele amplicon 8474 - KR35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR35: 5'-AAATCCCAAGGATGTTTCTAAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3547855
   
wt amplicon (over InsPos) 22km - KR35
wt amplicon start Chr3:3547855
wt amplicon end Chr3:3548774
wt amplicon size 920 bp
Locus specific primer KR35: 5'-AAATCCCAAGGATGTTTCTAAGGT-3'
2nd locus specific primer 22km: 5'-CTTCTCCGATCACAGTTTCTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11