Genotyping and primer details


Genotyping and primer details for 504E06 - At5g05730
Line ID 504E06
Locus At5g05730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1721906 of line 504E06 Chr5:1721906
   
Insertion allele amplicon 8474 - 61ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 61ra: 5'-CCACTTCATGAACAAGATAAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1721460
   
wt amplicon (over InsPos) 31rt - 61ra
wt amplicon start Chr5:1721460
wt amplicon end Chr5:1722283
wt amplicon size 824 bp
Locus specific primer 61ra: 5'-CCACTTCATGAACAAGATAAAGCA-3'
2nd locus specific primer 31rt: 5'-AAGCTCCTAGCTTTCTTTTCGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11