Genotyping and primer details

Genotyping and primer details for 524H06 - F13C5
Line ID 524H06
Locus F13C5 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10380356 of line 524H06 Chr4:10380356
Insertion allele amplicon 8474 - BF09
Locus specific primer BF09: 5'-AAAATTTTCAAATGGTTTGTGGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10379974
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11