Genotyping and primer details


Genotyping and primer details for 614A02 - At4g35040
Line ID 614A02
Locus At4g35040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16681494 of line 614A02 Chr4:16681494
   
Insertion allele amplicon 8474 - KC78
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KC78: 5'-TACTAATGCTGCTTGTACCCACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16680937
   
wt amplicon (over InsPos) 50rd - KC78
wt amplicon start Chr4:16680937
wt amplicon end Chr4:16682056
wt amplicon size 1120 bp
Locus specific primer KC78: 5'-TACTAATGCTGCTTGTACCCACAC-3'
2nd locus specific primer 50rd: 5'-CTCAAGTATGAAAACAAATGGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11