Genotyping and primer details


Genotyping and primer details for 622E02 - At1g04600
Line ID 622E02
Locus At1g04600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1262583 of line 622E02 Chr1:1262583
   
Insertion allele amplicon 8474 - AS30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AS30: 5'-ACAACCTAAGAAACAAATGCATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1262901
   
wt amplicon (over InsPos) AS30 - 02cu
wt amplicon start Chr1:1262095
wt amplicon end Chr1:1262901
wt amplicon size 807 bp
Locus specific primer 02cu: 5'-AAAAATCTGACCAAATTCCATCAT-3'
2nd locus specific primer AS30: 5'-ACAACCTAAGAAACAAATGCATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11