Genotyping and primer details

Genotyping and primer details for 637C09 - At2g32920
Line ID 637C09
Locus At2g32920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13962583 of line 637C09 Chr2:13962583
Insertion allele amplicon 8474 - KA20
Locus specific primer KA20: 5'-GTGGCATTGTCTTGTTCTCAGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13963110
wt amplicon (over InsPos) AJ23 - KA20
wt amplicon start Chr2:13962168
wt amplicon end Chr2:13963110
wt amplicon size 943 bp
Locus specific primer KA20: 5'-GTGGCATTGTCTTGTTCTCAGTTA-3'
2nd locus specific primer AJ23: 5'-GTCAATGATTGCCTGTTCATATTG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11