Genotyping and primer details


Genotyping and primer details for 658E02 - At4g26840
Line ID 658E02
Locus At4g26840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13497725 of line 658E02 Chr4:13497725
   
Insertion allele amplicon 8474 - AB12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB12: 5'-ATGTTATACCTCATCGGGAGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13498308
   
wt amplicon (over InsPos) 33hs - AB12
wt amplicon start Chr4:13497195
wt amplicon end Chr4:13498308
wt amplicon size 1114 bp
Locus specific primer AB12: 5'-ATGTTATACCTCATCGGGAGTTTG-3'
2nd locus specific primer 33hs: 5'-CCCATTATAACGAAACGACAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11