Genotyping and primer details


Genotyping and primer details for 703F03 - At1g78260
Line ID 703F03
Locus At1g78260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29448476 of line 703F03 Chr1:29448476
   
Insertion allele amplicon 8474 - NE08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NE08: 5'-CTAGGAGCTTGAAGAGAATAGCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29449041
   
wt amplicon (over InsPos) 05vu - NE08
wt amplicon start Chr1:29447752
wt amplicon end Chr1:29449041
wt amplicon size 1290 bp
Locus specific primer NE08: 5'-CTAGGAGCTTGAAGAGAATAGCCA-3'
2nd locus specific primer 05vu: 5'-CGGGAGGATTAGTTTATTTGATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11