Genotyping and primer details


Genotyping and primer details for 713C09 - At3g45240
Line ID 713C09
Locus At3g45240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16571144 of line 713C09 Chr3:16571144
   
Insertion allele amplicon 8474 - BG49
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BG49: 5'-TACATGGGTATTTCTTTCCCTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16571853
   
wt amplicon (over InsPos) 12em - BG49
wt amplicon start Chr3:16570684
wt amplicon end Chr3:16571853
wt amplicon size 1170 bp
Locus specific primer BG49: 5'-TACATGGGTATTTCTTTCCCTCTG-3'
2nd locus specific primer 12em: 5'-TATAACAAACTTCAAACGCAAACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11