Genotyping and primer details


Genotyping and primer details for 726G02 - At5g27210
Line ID 726G02
Locus At5g27210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9572559 of line 726G02 Chr5:9572559
   
Insertion allele amplicon 8474 - NF56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NF56: 5'-AGTCCTTACGGGTTTTACCTTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9573031
   
wt amplicon (over InsPos) 37gc - NF56
wt amplicon start Chr5:9572260
wt amplicon end Chr5:9573031
wt amplicon size 772 bp
Locus specific primer NF56: 5'-AGTCCTTACGGGTTTTACCTTCAT-3'
2nd locus specific primer 37gc: 5'-TGACTTCTAGGAGATCCTACCCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11