Genotyping and primer details


Genotyping and primer details for 765F10 - At4g15480
Line ID 765F10
Locus At4g15480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8850450 of line 765F10 Chr4:8850450
   
Insertion allele amplicon 8474 - BE07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE07: 5'-TGAACCTAGAAGAAGGATGGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8849851
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11