Genotyping and primer details


Genotyping and primer details for 796C09 - At3g61320
Line ID 796C09
Locus At3g61320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22694859 of line 796C09 Chr3:22694859
   
Insertion allele amplicon 8474 - 40lj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40lj: 5'-CTGATTCCACCTGTTTTCTTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22694344
   
wt amplicon (over InsPos) 13vu - 40lj
wt amplicon start Chr3:22694344
wt amplicon end Chr3:22695509
wt amplicon size 1166 bp
Locus specific primer 40lj: 5'-CTGATTCCACCTGTTTTCTTCTTC-3'
2nd locus specific primer 13vu: 5'-GGAAGACAACCTACATCACTCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11