Genotyping and primer details

Genotyping and primer details for 797D03 - At3g59030
Line ID 797D03
Locus At3g59030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21819715 of line 797D03 Chr3:21819715
Insertion allele amplicon 8474 - MZ76
Locus specific primer MZ76: 5'-TGGTCTCTGTTCTGAATTACATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21819314
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11