Genotyping and primer details


Genotyping and primer details for 797D11 - At3g57787
Line ID 797D11
Locus At3g57787 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21405018 of line 797D11 Chr3:21405018
   
wt amplicon (over InsPos) 58rd - CT27
wt amplicon start Chr3:21404741
wt amplicon end Chr3:21405429
wt amplicon size 689 bp
Locus specific primer CT27: 5'-TGATTACGTTATGGTTGGTGAAAA-3'
2nd locus specific primer 58rd: 5'-CATAAATGGGAAAATCTCCTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11