Genotyping and primer details


Genotyping and primer details for 941B08 - At2g31380
Line ID 941B08
Locus At2g31380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13382713 of line 941B08 Chr2:13382713
   
Insertion allele amplicon 8474 - PF02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF02: 5'-TTGTTGGGTACATTGGACTTCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13383235
   
wt amplicon (over InsPos) 22dd - PF02
wt amplicon start Chr2:13382280
wt amplicon end Chr2:13383235
wt amplicon size 956 bp
Locus specific primer PF02: 5'-TTGTTGGGTACATTGGACTTCATA-3'
2nd locus specific primer 22dd: 5'-GCCTTTTTCTTGACTCTCTCTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11