Genotyping and primer details


Genotyping and primer details for SALK_000947 - At1g67290
Line ID SALK_000947
Locus At1g67290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25192891 of line SALK_000947 Chr1:25192891
   
Insertion allele amplicon LBb3 - 60kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 60kh: 5'-ACCATGTGTCTGTTGTAAGCTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25192481
   
wt amplicon (over InsPos) 14ls - 60kh
wt amplicon start Chr1:25192481
wt amplicon end Chr1:25193378
wt amplicon size 898 bp
Locus specific primer 60kh: 5'-ACCATGTGTCTGTTGTAAGCTGTC-3'
2nd locus specific primer 14ls: 5'-AGAACCACCATACAAAATGAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11