Genotyping and primer details


Genotyping and primer details for SALK_000997c - At3g50520
Line ID SALK_000997c
Locus At3g50520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18748059 of line SALK_000997c Chr3:18748059
   
Insertion allele amplicon LBb3 - 25sr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25sr: 5'-CTTGTTGTCTTCCTGCATCATTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18748460
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11