Genotyping and primer details


Genotyping and primer details for SALK_001468c - At2g46470
Line ID SALK_001468c
Locus At2g46470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19072781 of line SALK_001468c Chr2:19072781
   
Insertion allele amplicon LBb3 - 21mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21mh: 5'-GGTTTTAAGGCCCATTAAGGTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19072240
   
wt amplicon (over InsPos) 08ng - 21mh
wt amplicon start Chr2:19072240
wt amplicon end Chr2:19073041
wt amplicon size 802 bp
Locus specific primer 21mh: 5'-GGTTTTAAGGCCCATTAAGGTTAG-3'
2nd locus specific primer 08ng: 5'-ATAGCAACCTCGTTTATAGCAGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11