Genotyping and primer details


Genotyping and primer details for SALK_001511c - At4g03960
Line ID SALK_001511c
Locus At4g03960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1888907 of line SALK_001511c Chr4:1888907
   
Insertion allele amplicon R204 - SC06
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC06: 5'-ATCGCAAATAGTCGCTGATTTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1887925
   
wt amplicon (over InsPos) 11lt - SC06
wt amplicon start Chr4:1887925
wt amplicon end Chr4:1889422
wt amplicon size 1498 bp
Locus specific primer SC06: 5'-ATCGCAAATAGTCGCTGATTTAGT-3'
2nd locus specific primer 11lt: 5'-TCCGAATTACGATTGAAGTCATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11