Genotyping and primer details


Genotyping and primer details for SALK_001525c - At1g21170
Line ID SALK_001525c
Locus At1g21170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7417030 of line SALK_001525c Chr1:7417030
   
Insertion allele amplicon LBb3 - 15ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15ea: 5'-TACTTGTTGTAAAGCTCAGAGGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7417747
   
wt amplicon (over InsPos) 13fg - 15ea
wt amplicon start Chr1:7416622
wt amplicon end Chr1:7417747
wt amplicon size 1126 bp
Locus specific primer 15ea: 5'-TACTTGTTGTAAAGCTCAGAGGCA-3'
2nd locus specific primer 13fg: 5'-CCCATAGTGCTGGTATTTGAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11