Genotyping and primer details


Genotyping and primer details for SALK_001615c - At3g62030
Line ID SALK_001615c
Locus At3g62030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22974526 of line SALK_001615c Chr3:22974526
   
Insertion allele amplicon LBb3 - 39dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39dh: 5'-CTAACCATGAAGTCTGCACAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22974996
   
wt amplicon (over InsPos) 16ec - 39dh
wt amplicon start Chr3:22974074
wt amplicon end Chr3:22974996
wt amplicon size 923 bp
Locus specific primer 39dh: 5'-CTAACCATGAAGTCTGCACAAAAA-3'
2nd locus specific primer 16ec: 5'-TTAAGCAATTTTCTGGGGTTTATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11