Genotyping and primer details


Genotyping and primer details for SALK_001778c - At5g56860
Line ID SALK_001778c
Locus At5g56860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22990478 of line SALK_001778c Chr5:22990478
   
Insertion allele amplicon LBb3 - 29gd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29gd: 5'-GGAGGGATCTTAAGAAGCCATATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22990894
   
wt amplicon (over InsPos) 09ho - 29gd
wt amplicon start Chr5:22989884
wt amplicon end Chr5:22990894
wt amplicon size 1011 bp
Locus specific primer 29gd: 5'-GGAGGGATCTTAAGAAGCCATATC-3'
2nd locus specific primer 09ho: 5'-CCATTGGAGGAGAGTGATTGTATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11