Sequences from confirmation amplicon


Confirmation sequences for line SALK_001778c and hit At5g56860 are shown.
Line ID SALK_001778c
Locus At5g56860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22990478
Primers used for PCR 29gd - LBb3, show primer details
   
Sequence Name 18-K112064-SALK_001778C-29gd
GenBank Accession LN484778 [GenBank]
Sequencing primer gene specific primer, 29gd: 5'-GGAGGGATCTTAAGAAGCCATATC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K112064-SALK_001778C-29gd
ACCTTGATCGGTGATATATATATACCAGGCCAAGATGTTTGTGGCTAACGGTGGATCATC
AGCATGCGATCACATGGTGCCAAAGAAGGAGACAAGACTGAAACTAACGATAAGGAAAAA
AGATCACGAAGACCAACCCCATCCTCTTCATCAAAACCCGACAAAACCCGATTCAGACTC
CGACAAGTGGTTGATGTCCCCAAAGATGCGGTTGATCAAGAAAACAATCACCAACAATAA
ACAGCTCATTGATCAGACTAATAATAATAATCATAAAGAAAGTGATCACTACCCTTTGAA
TCATAAGACTAATTTCGACGAGGATCACCATGAAGATCTTAATTTCAAGAACAAATTGAC
GCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 02-K112049-SALK_001778C-R210
GenBank Accession LN484305 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112049-SALK_001778C-R210
CATTTGTTCTTGAATTAGATCTTCATGGTGATCCTCGTCGAAATTAGTCTTATGATTCAA
AGGGTAGTGATCACTTTCTTTATGATTATTATTATTAGTCTGATCAATGAGCTGTTTATT
GTTGGTGATTGTTTTCTTGATCAACCGCATCTTTGGGGACATCAACCACTTGTCGGAGTC
TGAATCGGGTTTTGTCGGGTTTTGATGAAGAGGATGGGGTTGGTCTTCGTGATCTTTTTT
CCTTATCGTTAGTTTCAGTCTTGTCTCCTTCTTTGGCACCATGTGATCGCATGCTGATGA
TCCACCGTTAGCCACAAACATCTTGGCCTGGTATATATATATCACCGATCAAGGTTTATA
ATCAACAACAGATGGAATTGTACATAATAATATAATGATATGGCTTCTTAAGATCCCTC

Last Updated on 06.12.2018 16:11