Genotyping and primer details

Genotyping and primer details for SALK_002455c - At2g14690
Line ID SALK_002455c
Locus At2g14690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6284903 of line SALK_002455c Chr2:6284903
Insertion allele amplicon LBb3 - 28dr
Locus specific primer 28dr: 5'-GTGTGTTCTTTTGTGGAGATTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6285410
wt amplicon (over InsPos) 11mu - 28dr
wt amplicon start Chr2:6284356
wt amplicon end Chr2:6285410
wt amplicon size 1055 bp
Locus specific primer 28dr: 5'-GTGTGTTCTTTTGTGGAGATTTCA-3'
2nd locus specific primer 11mu: 5'-CAAACTAACTCAAACGTGCATTGT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11