Sequences from confirmation amplicon


Confirmation sequences for line SALK_002455c and hit At2g14690 are shown.
Line ID SALK_002455c
Locus At2g14690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6284903
Primers used for PCR 28dr - LBb3, show primer details
   
Sequence Name 05-K114130-SALK_002455C-R210
GenBank Accession LN484404 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114130-SALK_002455C-R210
CGTCGCATTTTCCCCGCGAGCTGCATCATTGAATCAGCTATTTTGTAGTTCTCTTGACCT
CTCACTGCCTCAGTTGCATACCATTTCATTTCATTGGTGAATGAAGTGAGTCTAAACCGC
GACACAAACCATTCTCTATAGCTATCGCTTTCCAAAATACGGTAGTTCATAGCACAACCA
AGGAGGAATGATGGTTTGATCTGTTCTATGGAAATCACTGATCCTTCTAATGCACTTTTG
TTCTTAAAACTCATTTGAAATCTCACTTTATTCTTCCGAATCTGCGAGTTTTTCTCAAAG
TCATAGATTGTTTTAAACGCTTATAAAACGTTTTCATAATGTATGTATAATACCTTTTCA
ATAACTTGGTCTTGTTGTAGCCTCCATTGAGTTTTGTGGAACCGTTGCATCCTCACATTT
TGTACTGATATCTCCAAACCCGCTAAGCCATCGCTCTGCTTCGTGTAAATTCCTACATTA
GTACTTGAAATCTCCACAAAGAAACACA
   
Sequence Name 15-K114131-SALK_002455C-28dr
GenBank Accession LN484699 [GenBank]
Sequencing primer gene specific primer, 28dr: 5'-GTGTGTTCTTTTGTGGAGATTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114131-SALK_002455C-28dr
ATTCCGAGCAAGCGATGGCTAGCGGGTTTGGAGATATCAGTACAAAATGTGAGGATGCAA
CGGTTCCACAAAACTCAATGGAGGCTACAACAAGACCAAGTTATTGAAAAGGTATTATAC
ATACATTATGAAAACGTTTTATAAGCGTTTAAAACAATCTATGACTTTGAGAAAAACTCG
CAGATTCGGAAGAATAAAGTGAGATTTCAAATGAGTTTTAAGAACAAAAGTGCATTAGAA
GGATCAGTGATTTCCATAGAACAGATCAAACCATCATTCCTCCTTGGTTGTGCTATGAAC
TACCGTATTTTGGAAAGCGATAGCTATAGAGAATGGTTTGTGTCGCGGTTTAGACTCACT
TCATTCACCAATGAAATGAAATGGTATGCAACTGAGGCAGTGAGAGGTCAAGAGAACTAC
AAAATAGCTGATTCAATGATGCAGCTCGCGGAGGAAAATGCGGACGTTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11