Genotyping and primer details


Genotyping and primer details for SALK_003000c - At1g78110
Line ID SALK_003000c
Locus At1g78110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29392903 of line SALK_003000c Chr1:29392903
   
Insertion allele amplicon LBb3 - 41us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41us: 5'-GAACAACAAAGATGACGACAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29392508
   
wt amplicon (over InsPos) 32vx - 41us
wt amplicon start Chr1:29392508
wt amplicon end Chr1:29393665
wt amplicon size 1158 bp
Locus specific primer 41us: 5'-GAACAACAAAGATGACGACAAGAA-3'
2nd locus specific primer 32vx: 5'-AACTTTGTTCATGGACATTTGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11