Genotyping and primer details


Genotyping and primer details for SALK_003192 - At3g13970
Line ID SALK_003192
Locus At3g13970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4613828 of line SALK_003192 Chr3:4613828
   
Insertion allele amplicon LBb3 - 03rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03rb: 5'-TCGTGTCTATGGTTTTCTTTTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4614608
   
wt amplicon (over InsPos) 26rz - 03rb
wt amplicon start Chr3:4613425
wt amplicon end Chr3:4614608
wt amplicon size 1184 bp
Locus specific primer 03rb: 5'-TCGTGTCTATGGTTTTCTTTTCCT-3'
2nd locus specific primer 26rz: 5'-TCATCGACTATCAATCACATCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11