Sequences from confirmation amplicon


Confirmation sequences for line SALK_003192 and hit At3g13970 are shown.
Line ID SALK_003192
Locus At3g13970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4613828
Primers used for PCR 03rb - LBb3, show primer details
   
Sequence Name 64-K116055-SALK_003192C-03rb
GenBank Accession LN485854 [GenBank]
Sequencing primer gene specific primer, 03rb: 5'-TCGTGTCTATGGTTTTCTTTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K116055-SALK_003192C-03rb
GTGCTCCGATACTGAAAAATCTAAATTCAAGGTATATCTTCTTTTGTAATCAATCATTCC
TTCCTTTCGCGAATCACCAAACTTAATCTATGAGATGAAATTAGGGTTCTTATGTGTATT
GGAACCTTCCATTAGCGATATAGAACACTGTCAAGGATATGATTAGATGATTTTTTTACT
GAGATCTTCAGTTGATACAATGTATGTGTGTTTGTTTAACCACGATATTTTATTTACGAG
AAATCCACCAGTTTGTTCTTCTTTGGATTTTGTTGGTTGATAGGAAACTTCGTTAGCATT
GTCTCTGGATCAACTAGAACTAGGCATTAGGCAACATCATAATCATTAGCTTTGTAATTA
GTTATAATCTTTTGATTATTAGCTTTGTTATATATGTGCTCTGCTTTGGTGAGTTCAGGT
TTCAGGGAGTGACAAGTTTGCTAACGTGATTGATTTTCTAAGACGGCAGCTTCACTCTGA
CTCATTGGTAATCATTTATCGATATCTTCTGTGTTTATCTCTTGTGTAACATTGGTTTTG
CCGCAAGTGTGCTGATATCTCTCCACTGTGTTAGTTTGTTTATGTGAACAGCGCTTTCTC
GCCAAACCCGGATGAATCAGTAATTGATCTATACAATGCAAGTATTTTCACTATTTCATT
CGTACCATGCTCCTGAAACTAGATATACATCGTCAAGCATGGCTTGTTTGATCTTAATAT
TGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATC
CTGTTTGATGGTGGTTC
   
Sequence Name 37-K116054-SALK_003192C-R210
GenBank Accession LN485253 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116054-SALK_003192C-R210
TTTGTTACCCACAATATTAAGATCAACAAGCCATGCTTGACGATGTATATCTAGTTTCAG
GAGCATGGTACGAATGAAATAGTGAAAATACTTGCATTGTATAGATCAATTACTGATTCA
TCCGGGTTTGGCGAGAAAGCGCTGTTCACATAAACAAACTAACACAGTGGAGAGATATCA
GCACACTTGCGGCAAAACCAATGTTACACAAGAGATAAACACAGAAGATATCGATAAATG
ATTACCAATGAGTCAGAGTGAAGCTGCCGTCTTAGAAAATCAATCACGTTAGCAAACTTG
TCACTCCCTGAAACCTGAACTCACCAAAGCAGAGCACATATATAACAAAGCTAATAATCA
AAAGATTATAACTAATTACAAAGCTAATGATTATGATGTTGCCTAATGCCTAGTTCTAGT
TGATCCAGAGACAATGCTAACGAAGTTTCCTATCAACCAACAAAATCCAAAGAAGAACAA
ACTGGTGGATTTCTCGTAAATAAAATATCGTGGTTAAACAAACACACATACATTGTATCA
ACTGAAGATCTCAGTAAAAAAATCATCTAATCATATCCTTGACAGTGTTCTATATCGCTA
ATGGAAGGTTCCAATACACATAAGAACCCTAATTTCATCTCATAGATTAAGTTTGGTGAT
TCGCGAAAGGAAGGAATGATTGATTACAAAAGAAGATATACCTTGAATTTAGATTGTTTC
AGTATCGGAGCACCACCAGTAGCTCGCAGATGAACAACAACTGCGACGAAATTAAGGAAA
AGAAAACCATAGACACGA

Last Updated on 06.12.2018 16:11