Genotyping and primer details


Genotyping and primer details for SALK_003425c - At1g62020
Line ID SALK_003425c
Locus At1g62020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22920455 of line SALK_003425c Chr1:22920455
   
Insertion allele amplicon LBb3 - 28de
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28de: 5'-CCAAATTAACGCAAAAGAGAGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22919536
   
wt amplicon (over InsPos) 12lt - 28de
wt amplicon start Chr1:22919536
wt amplicon end Chr1:22921006
wt amplicon size 1471 bp
Locus specific primer 28de: 5'-CCAAATTAACGCAAAAGAGAGAAC-3'
2nd locus specific primer 12lt: 5'-TCATGTCCTGCTGCTAGTAGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11